Punching holes in membranes!

Using time‐lapse AFM at high temporal and spatial resolution, Estafania Mulvihill unravelled the mechanisms of how gasdermin-D assembles and forms pores in lipid membranes. Dynamic structures of arc‐ and slit‐shaped protein oligomers that transform into larger thermodynamically stable ring‐shaped oligomers were observed. The insights highlight the mechanism by which gasdermin-D self‐assembles and forms transmembrane pores.